Composite

Part:BBa_K2557026:Design

Designed by: Ge JT   Group: iGEM18_NAU-CHINA   (2018-10-10)


EF1α promoter--TP901-RDF


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 1810
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 1810
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 568
    Illegal BglII site found at 869
    Illegal BglII site found at 1463
    Illegal BglII site found at 1589
    Illegal BglII site found at 1816
    Illegal BamHI site found at 220
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 1810
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 1810
    Illegal AgeI site found at 226
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1066
    Illegal SapI.rc site found at 2010


Design Notes

In order to allow the recombinase to enter the nucleus and function, we added SV40 NLS to the front end. To detect protein content, we added the FLAG tag.


Source

The EF1α promoter was cloned from a plasmid borrowed from other laboratories in the school. We found the sequence of TP901-RDF from the literature and synthesized this sequence.

References